ISHS Acta Horticulturae 706: IV International Symposium on Brassicas and XIV Crucifer Genetics Workshop
TOWARDS THE CLONING OF CLUBROOT RESISTANCE GENE (CRB) IN CHINESE CABBAGE |
| Authors: | Z.Y. Piao, W.C. Lee, Y.K. Lee, H.G. Kim, J.Y. Jeong, C.H. Hwang, Y.P. Lim |
| Abstract:
Clubroot disease, caused by Plasmodiophora brassicae Wor., is a highly damaging disease of Chinese cabbage. The CR Shinki DH line of Chinese cabbage carries a single dominant gene (CRb) for resistance. An F2 population was analyzed using developed DNA markers, and a genetic map around the CRb locus covering a total distance of 6.75 cM was constructed. Using three nearest surrounded markers (TCR01, TCR05, and TCR09), 17 recombinants were detected in a segregating F3 population (487 individuals). Among them, 11 were between TCR09 and CRb and five between CRb and TCR05, respectively. To construct a saturated map around CRb, the AFLP technique was employed by using 256 EcoR I/Mse I and 240 Sno I/Mse I primer combinations. Though, it was not possible to identify more closely linked markers in EcoR I/Mse I, yet among 240 Sno I/Mse I was tested in a bulked segregant analysis, and 8 closely linked markers were identified. Proteomic analysis of root tissues indicated that 5 protein spots were up-regulated; eight down-regulated; eight were new; and four not found in CR Shinki DH line as compared with 94SK. Biotinylated cRNA synthesized from CR Shinki DH line after inoculation with clubroot was hybridized to the oligonucleotide array containing probes against more than 24,000 genes for A. thaliana. The gene expression analysis indicated that only 4,748 genes were expressed. Among them, 1,232, 703, 950, 769, and 1,094 genes were from chromosome 1, 2, 3, 4, and 5 of A. thaliana, respectively. Based on the change of expression more than two folds, 9 genes are down regulated and 26 are up regulated two days after inoculation. | |
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