ISHS Acta Horticulturae 706: IV International Symposium on Brassicas and XIV Crucifer Genetics Workshop
ESTABLISHMENT OF AN INTEGRATED MARKER SYSTEM FOR OILSEED RAPE BREEDING (IMSORB) |
| Authors: | L. Bancroft, S. Barnes, J.Y. Li, J.L. Meng, D. Qiou, Schmidt, M. Trick, J. Wilmer |
| Keywords: | Brassica, genetic markers, Cruciferae genomics |
| Abstract:
Oilseed rape (Brassica napus) is widely grown in Europe and Asia as a cash crop. The oil extracted from its seeds has important food and non-food uses, including potential as a biofuel. We aimed to address some of the obstacles to the development of cultivars adapted to specific environmental conditions by integrating genetic and genomic resources. In doing so, we established new strategies for the development of sequence-based markers for use in B. napus that are targeted to specific loci in the genome that are themselves integrated with Brassica physical maps and the Arabidopsis genome sequence. Important insights were gained into the extent of sequence divergence between homoeologous loci within the B. napus genome, and of allelic sequence divergence. Marker assay systems, with low consumables costs, were developed and reduced to practice for InDel, CAPS and SNP marker types. A genetic linkage map was developed for a recently developed mapping population. | |
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