ISHS Acta Horticulturae 706: IV International Symposium on Brassicas and XIV Crucifer Genetics Workshop
INTERGENERIC TRANSFER OF NEMATODE RESISTANCE FROM RAPHANUS TO BRASSICA USING A SERIES OF RAPE-RADISH CHROMOSOME ADDITION LINES |
| Authors: | H. Budahn, H. Peterka, O. Schrader, S. Zhang |
| Keywords: | wide hybridization, Heterodera schachtii, FISH, RAPD |
| Abstract:
In the genus Raphanus genetic resistance is present against the beet cyst nematode (Heterodera schachtii). Transfer of nematode resistance to rapeseed is desirable to convert this tolerant host plant into a resistant crop. Nematode-resistant winter rapeseed would be of interest as an agronomically important trap crop in rotations with sugar beet. Starting with the initial crosses oil radish x cabbage (RRRR x CCCC) and fodder radish x chinese cabbage (RRRR x AA), a trigenomic hybrid (RRAC) was developed and crossed several times with oilseed rape (AACC). This hybridization programme resulted in a basic population in which the presence of the nine individual radish chromosomes, a to i, was detected by cytogenetic and molecular methods (Peterka et al., 2004). The nematode resistance was localized to chromosome d. After a further cross to rape, we were able to identify for each radish chromosome monosomic addition lines (MAL’s) having 38 chromosomes of oilseed rape and one individual chromosome of radish (2n = 4x = 38 + 1; MAL a to i). The added chromosomes in the nine MAL’s were cytologically detected with fluorescence in situ hybridization (FISH) using a radish-specific probe. The MAL d was crossed with rape. The progenies were classified with chromosome-specific RAPD/double-primer RAPD markers for presence/absence of the extra chromosome. They were inoculated with L2 juveniles of Heterodera schachtii and cultivated under controlled conditions. The MAL of chromosome d had the same resistance level as the radish variety used as chromosome donor whereas the plants without the radish chromosome were as susceptible as the rapeseed recipient. | |
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