ISHS Acta Horticulturae 705: V International Walnut Symposium
PLANTLET REGENERATION FROM EXCISED EMBRYONAL AXES, SHOOT APICES AND NODAL SEGMENTS OF JUGLANS REGIA L. |
| Authors: | A. Fatima, A.N. Kamili, A.M. Shah |
| Keywords: | embryonal, nodal segments, regeneration |
| Abstract:
Embryonal axes, in vitro raised shoot apices and nodal segments of Walnut (Juglans regia) were cultured on MS medium (Murashige and Skoog, 1962) supplemented with different concentrations and combinations of plant growth regulators. The varying response of cultured embryos to various phytohormones was observed and the best results were obtained when combination of BAP and IAA was used. Stimulation and development of multiple shoot buds (6-8) on both sides of embryonal axes was observed after 10 weeks of culture on MS (x1/2) augmented with IAA (11.4µM) + BAP (8.8 µM), where as use of full strength MS medium supplemented with same hormonal concentrations revealed same results but only after 8 weeks of culture period. Shoot apices and nodal segments from in vitro raised seedlings were cultured on various hormonal concentrations and showed fantastic results. 18 multiple shoots were observed on medium supplemented with IAA (11.4 µM) + BAP (8.8 µM). Multiplication continued for months together provided the material was transferred to fresh medium after every 4-6 weeks or else the proliferation would decrease. Remarkable elongation of these micro shoots was noticed after 8-10 weeks of culture when combination of auxin and cytokinin was used. Different trials for rooting made by culturing these in vitro raised elongated shoots on different rooting media resulted in 6 well developed root initials at cut ends after 6-8 weeks of culture period. | |
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