ISHS Acta Horticulturae 705: V International Walnut Symposium
PCR-RFLP ANALYSIS OF CP-DNA IN THE GENUS JUGLANS |
| Authors: | M. Abuin, R. Diaz, J. Alfonsin, N. Aleta, A. Ninor, J. Fernández-López |
| Keywords: | chloroplast DNA, Juglans section, species-specific molecular markers, phylogeny |
| Abstract:
In this work we used the PCR-RFLP analysis of chloroplast DNA to determine if this method could be employed to detect inter-specific variation in the genus Juglans (family Juglandaceae). Different chloroplast DNA regions were amplified with specific primers in five species of Juglans: J. regia, J. nigra, J. major, J. hindsii and J. australis, and digested with several restriction enzymes in order to detect interspecific polymorphisms. Species-specific restriction fragment length polymorphisms patterns were determined for three species: J. regia, J. major and J. hindsii. Intra-specific variation was also found in two of the species analysed, J. australis and J. nigra. Analysis of different Juglans hybrids specimens indicated that the chloroplast genome was maternally inherited in this genus and therefore appropriate to determine maternal phylogenetic relationships. From a UPGMA and NJ cluster analysis based on a simple matching similarity matrix the Persian walnut species was clearly distinguished from the four taxa of American walnuts. Within black walnuts, J. nigra from Northwest America showed a haplotype that differed substantially from J. major, J. hindsii and J. australis, which suggest that these last species may have a common maternal origin and may be distinct from J. nigra | |
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