ISHS Acta Horticulturae 705: V International Walnut Symposium
MOLECULAR CHARACTERIZATION OF JUGLANS REGIA L. CULTIVARS WITH SSR MARKERS |
| Authors: | I. Foroni, R. Rao, K. Woeste |
| Keywords: | walnut, diversity, Juglans nigra, cultivar identification, genetic relationships |
| Abstract:
DNA microsatellites are abundant, uniformly distributed, hypervariable, codominant and highly reproducible. They are a powerful and informative method to study genetic relationships and genotype identity. We used nine microsatellites (WGA4, WGA33, WGA80, WGA147, WGA148, WGA204, WGA221, WGA256, WGA275) originally isolated in black walnut (Juglans nigra L.) to characterize six Persian walnut cultivars; four from Europe (‘Malizia’, ‘Blegiana’, ‘Parisienne’ and ‘Franquette’) and two from the U.S. (‘Serr’ and ‘Hartley’). The microsatellites amplified a total of 43 putative alleles. The number of alleles per locus ranged from four to eight, with a mean of 5.3, and a molecular size range from 130 bp to 296 bp. The eight microsatellite loci were sufficiently polymorphic that unique genotypes for each cultivar were easily discernable. There are many potential applications of microsatellites to walnut genetics, including pedigree analysis, breeding, population genetics, germplasm management, pollen flow analysis, and cultivar identification. | |
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