ISHS Acta Horticulturae 704: X International Workshop on Fireblight
PHENOTYPIC AND GENETIC DIVERSITY OF SELECTED ERWINIA AMYLOVORA STRAINS FROM POLAND |
| Authors: | J. Pulawska, K. Kielak, P. Sobiczewski |
| Keywords: | PCR-RFLP, RAPD, virulence |
| Abstract:
To determine the diversity of Erwinia amylovora occurring in Poland, 14 strains originated from six species of host plants growing in different geographical regions were characterized on the basis of their phenotypic and genetic features. The virulence of strains was tested on apple leaves and shoots of apple, pear and hawthorn. The majority of strains isolated from fruit trees appeared to be most virulent, whereas 3 out of 4 least virulent strains originated from pyracantha, cotoneaster and mountainash. In spite of significant differences in virulence, all tested strains were very homogeneous with regard to their phenotypic features determined with API 50CH, API ZYM and API 20NE kits. High homogeneity was also observed in results of DNA-based analyses. Identical patterns were obtained in RFLP analysis of amsB gene amplified earlier with primers AMSbL and AMSbR. In the case of RAPD, a discrete DNA polymorphism was only found within the studied strains with one out of the five primers tested. | |
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