ISHS Acta Horticulturae 704: X International Workshop on Fireblight
AUTOINDUCTION IN ERWINIA AMYLOVORA |
| Authors: | L. Molina, G. Defago, B. Duffy |
| Keywords: | EPS, fire blight, gene regulation, LuxI, LuxR, N-acyl homoserine lactone, quorum sensing |
| Abstract:
Erwinia amylovora produces a metabolite acting as an autoinducer that can be detected by standard biosensor assays. Genetic analysis revealed high homology of the newly described eamI/eamR genes with N-acyl-homoserine lactone (AHL) signal/receptor of other pathogenic bacteria coded by the luxI/luxR genes. Further proof of existence of an AHL-type signal in E. amylovora was provided by constructing an heterologous expression model with the Bacillus sp. A24 acyl-homoserine lactonase gene aiiA. E. amylovora expressing the gene aiiA produced less of EPS (amylovoran and levansucrase) which is a virulence factor, had a reduced tolerance to free oxygen radicals (H2O2), and reduced virulence on apple (detached leaf assay in vitro). | |
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