ISHS Acta Horticulturae 704: X International Workshop on Fireblight
HRPL NEGATIVELY REGULATES FLAGELLAR SYSTEM IN ERWINIA AMYLOVORA |
| Authors: | S. Cesbron, M.N. Brisset, M. Tharaud, J.P. Paulin |
| Keywords: | Erwinia amylovora, hrpL, flagellin, motility |
| Abstract:
Several previous works seem to indicate that flagellar and hrp genes of Erwinia amylovora are counter-regulated. In the present paper, this hypothesis is confirmed by the analysis of several parameters linked to flagella synthesis in E. amylovora grown in hrp- or non hrp-inducing conditions. Moreover, amongst the various hrp mutants tested, the hrpL regulatory mutant only was more motile, produced more flagellins and showed more flagella than the wild-type strain when cultivated on a hrp-inducing medium. A plasmid carrying hrpL introduced into a hrpL mutant decreased its motility and flagellin production. These results strongly suggest that flagellar synthesis of E. amylovora is under the negative control of HrpL, a positive regulator of hrp genes. | |
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