ISHS Acta Horticulturae 703: II International Symposium on Sweetpotato and Cassava: Innovative Technologies for Commercialization
SWEETPOTATO NODAL CULTURE, PLANTLET GROWTH UNDER PHOTOMIXOTROPHIC CONDITIONS AND CONFIRMATION OF CYTOGENETIC STABILITY |
| Authors: | D.T.T. Giang, M. Tanaka, J.A. Teixeira da Silva |
| Keywords: | flow cytometry, photoautotrophy, RAPDs, somaclonal variation |
| Abstract:
In order to overcome the limitations of growth imposed by conventional tissue culture vessels, the newly developed gas-permeable “Vitron” film vessel was applied in the photomixotrophic micropropagation of sweetpotato using nodes 4 and 5 (confirmed to have the highest regeneration capacity and to be cytogenetically stable). Photoautotrophic cultures (CO2-enrichment, sugar-free) of leafless single nodes induce shoots poorly. Moreover, the photoheterotrophic (sucrose-enriched medium) culture of nodes of sweetpotato plants on agar-based medium in conventional, closed vessels (polycarbonate) have been shown to be inferior to that of plantlets grown on the same medium in the Vitron under photomixotrophic conditions. Consequently, to further characterize the importance of sucrose and CO2 level on the effectiveness of photomixotrophic culture, the present study was conducted. Despite differences in RAPD banding, acclimatized plants did not exhibit any marked phenotypic differences. Cytological (flow cytometry) analyses demonstrated that in vitro and ex vitro cultures were very stable, exhibiting no (endo)polyploidy. | |
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