ISHS Acta Horticulturae 701: XII International Symposium on Apricot Culture and Decline
VIRUSES AND CERTIFICATION OF APRICOT TREES IN BASILICATA REGION (SOUTHERN ITALY) |
| Authors: | A. Crescenzi, A. Fanigliulo, S. Comes, R. Pacella, P. Piazzolla, V. Vitelli |
| Keywords: | Prunus armeniaca, PPV, ApMV, ACLSV, PNRSV, PDV |
| Abstract:
Apricot represents one of the most important stone fruits species in Basilicata Region (Southern Italy). In order to assess the presence and distribution of the main viruses affecting it, surveys were carried out from 1999 through 2001 on apricot orchards in the main producing areas of the Region. Analyses were performed in order to detect the presence of plum pox virus (PPV, genus Potyvirus, family Potyviridae), prunus necrotic ring spot virus (PNRSV, genus Ilarvirus, family Bromoviridae), apple mosaic virus (ApMV, genus Ilarvirus, family Bromoviridae), prune dwarf virus (PDV, genus Ilarvirus, family Bromoviridae) and apple chlorotic leaf spot virus (ACLSV, genus Tricovirus), and were carried out by a serological assay, DAS-ELISA. In order to determine the PPV subgroup, PPV positive samples were assayed using PPV-C, D, M, and EA subgroup specific monoclonal antibodies, and by RT-PCR followed by RFLP analysis. Results concerning the above mentioned viruses are reported in the present work. A certification scheme for apricot was formally established on 14th April 1997 by a Decree of the Italian Ministry of Agriculture and Forestry. It concerns different pathogens affecting stone fruits, among which PNRSV, PDV, ApMV and ACLSV. In order to start the C.A.C. (Conformitas Agraria Comunitatis) certification, surveys and tests to select mother plants with a sanitary status compatible with the requirements of the scheme were carried out from 1999, leading to the production of about 400,000 certified apricot scions. | |
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