ISHS Acta Horticulturae 701: XII International Symposium on Apricot Culture and Decline
COMPARISON OF TWO METHODS OF ARTIFICIAL INOCULATION IN THE EVALUATION OF APRICOT GENOTYPES FOR RESISTANCE TO PLUM POX VIRUS |
| Authors: | M. Glasa, O. Kudela, Z. Glasova, D. Benedikova |
| Keywords: | sharka, Prunus armeniaca, breeding, detection |
| Abstract:
The reaction of 12 Slovak apricot cultivars and hybrids (breeding program of Research Breeding Station at Veselé) to infection by M-type isolates of Plum pox virus (PPV-M) was evaluated. Two methods of the artificial inoculation were compared: grafting of apricots on naturally infected plum trees and chip-budding of apricots previously grafted on healthy GF 305 seedlings in the glasshouse. Monitoring of PPV infection was done for 3-year period by symptomatology, DAS-ELISA and RT-PCR. Final evaluation of field and glasshouse experiments showed that method of inoculation had no significant influence on the reaction of genotypes to PPV infection, although a delay of PPV expression and minor discordance in symptom presence could be noted in some genotypes. Four Slovak apricot genotypes (Veharda, Vemina, VS 158/1 and VS 157/8) proved to be resistant to PPV-M infection in both tests. | |
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