ISHS Acta Horticulturae 701: XII International Symposium on Apricot Culture and Decline
NATURAL POPULATION OF RECOMBINANT PLUM POX VIRUS OCCURS IN SLOVAKIA |
| Authors: | M. Glasa, O. Kudela, V. Marie-Jeanne, G. Labonne, F. Dosba, J.B. Quiot |
| Keywords: | potyvirus, recombination, epidemiology, variability, Prunus, sharka |
| Abstract:
The Slovak isolate BOR-3, collected from an apricot tree in 1996, was identified as a natural PPV recombinant between an M and D type of Plum pox virus. Several assays were conducted to evaluate the biological properties of this isolate. The recombinant isolate was transmitted to Prunus insititia × P. domestica St. Julien no. 2 (causing intensive chlorosis), P. armeniaca cv. Manicot (leaf necrosis) and P. persica GF305 (symptomless reaction). Its capacity to be transmitted by aphids was experimentally confirmed. The isolate could be easily maintained in vitro on several susceptible Prunus spp. hosts. Closely related recombinant variants were detected in different Prunus in the initial focus in the spring of 2001. Sequence analysis showed high homology between BOR-3 and newly sequenced isolates, reaching more than 99%. All the Slovak recombinant isolates share the same recombination breakpoint. In addition, recombinants showed a close serological relationship, although the serological pattern differed from that of other Slovak PPV isolates. This demonstrates that the recombinants are viable, competitive with conventional PPV-M and D isolates and circulates throughout this locality probably via aphid activity. The present work indicates that the occurrence of recombinants within PPV isolates might be more common then previously assumed. | |
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