ISHS Acta Horticulturae 701: XII International Symposium on Apricot Culture and Decline
GENETIC LINKAGE MAPS OF TWO APRICOT CULTIVARS (PRUNUS ARMENIACA L.) BASED ON RAPD AND AFLP MARKERS |
| Authors: | M.A. Hurtado, G. Llacer, M.L. Badenes, A.G Abbott |
| Keywords: | Prunus armeniaca, molecular markers |
| Abstract:
Genetic linkage maps for two apricot cultivars have been constructed using RAPD and AFLP markers in 81 F1 individuals from the cross ‘Goldrich’ x ‘Valenciano’. One hundred sixty RAPD arbitrary primers were screened and a total of 44 were selected. A total of 67 polymorphic RAPD markers were scored on the mapping population: 30 were heterozygous in ‘Goldrich’, 19 were heterozygous in ‘Valenciano’, segregating 1:1 and 12 markers heterozygous in both parents segregating 3:1. Forty primer combinations were used for AFLP and all of them detected polymorphism. A total of 143 AFLP polymorphic markers were identified. Of the 98 markers segregating 1:1, 65 were heterozygous in the female parent (Goldrich) and 32 in the male parent 'Valenciano'. Forty-five markers were present in both parents and segregated 3:1. A total of 117 markers were placed into 8 linkage group on the 'Goldrich' map, defining 570.3 cM of total map length. The average distance between adjacent markers was 4.9 cM. A total of 66 markers were placed into 9 linkage groups on the 'Valenciano' map, defining 448.9 cM of total map distance, with an average interval of 6.6 cM between adjacent markers. Twelve markers loci heterozygous in both parents revealed straightforward homologies between 4 linkage groups in both maps. A segregation-ratio distorted fragments of 9 % and 17 % was found for RAPD and AFLP markers respectively. | |
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