ISHS Acta Horticulturae 701: XII International Symposium on Apricot Culture and Decline
SEASONAL PROTEIN VARIATIONS IN APRICOT BARK |
| Authors: | M. Faurobert, L. Gomez |
| Keywords: | Prunus armeniaca, vegetative storage proteins, nitrogen store, parenchyma, phloem, two-dimensional electrophoresis |
| Abstract:
In a previous work on Peach tree we have shown that bark tissues contain specific proteins which undergo seasonal fluctuations. These proteins are present in large quantities during the winter and are merely absent during the summer. In Apricot we compared phloem and parenchyma of four different varieties in January and June. Analysis of total nitrogen, amino acid content, protein content, protein patterns by SDS-page (1DE) and two dimensional electrophoresis (2DE) are presented. Some great differences were detected between the two periods for all the varieties tested. In 1DE three low MW protein bands were clearly accumulated in winter. Two of these bands had MW (19000 and 16500 kd) closed those of peach vegetative storage proteins, although the third one (18000 kd) was not previously detected in peach. The polypeptides concerned by theses variations were characterised by 2DE. We could demonstrate that several polypeptidic spots, were implicated in the variation of each band of the 1DE pattern. Our results point out which bark proteins are susceptible to play a major role in N supply in the spring. | |
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