ISHS Acta Horticulturae 701: XII International Symposium on Apricot Culture and Decline
PHYSIOLOGICAL AND GENETIC STUDIES ON APRICOT/PRUNUS ROOTSTOCKS GRAFT COMPATIBILITY |
| Authors: | J.L. Poëssel, M. Faurobert, M. Loonis, M.N. Corre, G. Olivier, V. Restier, J.M. Audergon, M. Masse |
| Keywords: | rootstock breeding, bark, cyanogenic compounds, phenolic compounds, prunasin, prunin, proteins, 2D PAGE electrophoresis, biochemical markers |
| Abstract:
Graft compatibility is a main criterion for apricot rootstock selection. Localised graft incompatibility shown by many apricot cultivars when grafted on different Prunus rootstocks greatly reduces the range of available rootstocks for apricot cultivation. A multidisciplinary research program has been developed at INRA Avignon on the genetic and physiological basis of graft incompatibility. The biochemical comparison of compatible and incompatible apricot cultivars showed the high level and the great variations of secondary compounds in the bark, including cyanogenic compounds and phenolics. Nevertheless, no relationship between prunin or prunasin content and graft compatibility of cultivars was observed. Proteome analysis by 2D PAGE electrophoresis showed that some constituent proteins of leaves could be good candidates as compatibility markers. Moreover, important changes in the protein content and in the protein two-dimensional electrophoresis maps occurred in the rootstock bark when grafted with an incompatible cultivar. The genetic determinism of compatibility is being analysed on a diallel cross between apricot cultivars showing contrasted compatibility phenotypes. The first results are presented. | |
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