ISHS Acta Horticulturae 698: VI International Symposium on Chemical and non-Chemical Soil and Substrate Disinfestation - SD2004
HORTICULTURE BECOMES ´HIGH TECH´ - MULTIPLEX DETECTION AND IDENTIFICATION OF PLANT PATHOGENS BY DNA ARRAYS |
| Authors: | B. Lievens, A.C.R.C. Vanachter, M. Brouwer, B.P.A. Cammue, B.P.H.J. Thomma |
| Keywords: | molecular diagnostics, macroarrays, microarrays, PCR, real-time PCR |
| Abstract:
Accurate detection and measurement of both plant pathogenic and beneficial microorganisms forms the basis of environmentally sound plant health management. The lack of rapid, accurate and reliable means by which plant pathogens can be detected and identified has been one of the main limitations in integrated disease management. Several molecular detecting techniques can generate accurate results rapidly enough to be useful for disease management decisions. However, if different microorganisms need to be detected simultaneously in a single assay, DNA array technology is currently the most suitable and most cost effective technique. Here, we highlight some of the recent developments in diagnostics that are likely to impact future plant disease controlling and preventing strategies. | |
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