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| Authors: | A.K. Gupta, Sonali, R.C. Sharma, K. Khosla |
| Keywords: | seedling blight, apple, Sclerotium rolfsii, eco-friendly approaches |
Abstract:
In recent years, seedling blight disease caused by Sclerotium rolfsii Sacc. has emerged as a serious constraint in raising healthy apple nursery plants.
In the present investigation eco-friendly strategies like organic amendments and bio-control agents were evaluated under in vitro conditions, pot culture conditions and field conditions.
Maximum reduction (77.41%) in radial growth of the pathogen was observed in Bacillus sp. strain 1 followed by Trichoderma viride (72.22%) under in vitro conditions.
In vitro evaluation of different plant materials and neem oil formulation further revealed hundred per cent inhibition in sclerotial germination in mustard cake + neem oil, neem cake + deodar needles + neem oil and mustard cake + neem cake + pine needles + neem oil treatments.
Under pot culture conditions, T. viride alone, and its combination with neem oil + mustard cake and neem oil + neem cake + deodar needles provided hundred per cent disease control. Trichoderma viride when applied 15 days prior to inoculation of pathogen; at the time of inoculation and 15 days after inoculation of pathogen as wheat bran - saw dust preparation (280 g/m2) was found most effective under field conditions resulting in 2.78 and 2.64 per cent disease incidence as compared to 87.56 and 92.67 per cent in untreated nursery beds in the years 2000 and 2001, respectively, and enhanced the growth of apple seedlings.
Talc based formulation of T. viride tested under field conditions in the year 2002 also caused remarkable reduction (93.58%) as compared to 68.88 per cent in usually recommended thiram treated nursery beds.
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