ISHS Acta Horticulturae 696: VII International Symposium on Temperate Zone Fruits in the Tropics and Subtropics - Part Two
MOLECULAR CHARACTERIZATION AND GENETIC DIVERSITY IN FRAGARIA GENOTYPES AS REVEALED BY RANDOMLY AMPLIFIED DNA POLYMORPHISMS (RAPDS) |
| Authors: | S. Kashyap, R. Kaur, D.R. Sharma, K. Kumar, S.K. Sharma |
| Keywords: | strawberry, DNA fingerprinting, genetic relatedness, cluster analysis |
| Abstract:
The efficiency of RAPD markers for Fragaria genotype DNA fingerprinting and the estimation of genetic relatedness was evaluated. Thirteen cultivars of Fragaria × ananassa Duch. and two wild species, Fragaria indica Andr. and Fragaria vesca Linn. were analyzed with twenty 10-bp primers. Only thirteen yielded scorable polymorphic amplification patterns based on discernible bands, however, no single primer out of these thirteen could produce a unique fingerprint for all the fifteen genotypes. Cluster analysis based on UPGMA separated the fifteen Fragaria genotypes into two major groups, with the thirteen cultivars and Fragaria indica in a group separate from Fragaria vesca. The mean genetic distance among the genotypes was 36 % implying that large part of the genome is not similar among the genotypes. Genetic diversity studies could not be related to pedigrees due to a lack of information about the same. | |
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