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ISHS Acta Horticulturae 695: I International Symposium on Tomato Diseases

RESISTANCE TO VERTICILLIUM DAHLIAE RACE 2 AND ITS INTROGRESSION INTO PROCESSING TOMATO CULTIVARS

Author:   L. Stamova
Keywords:   Lycopersicon esculentum, disease resistance, breeding
Abstract:
A race of Verticillium dahliae, virulent on cultivars with the Ve gene, designated race 2, was detected in 1957 and later reported in North and South America, Europe, Africa and Australia. Until recently the screenings of germplasm have not revealed a reliable source of resistance to race 2. The objective of this work was to confirm the resistance to race 2 and the mode of its inheritance in the author’s line Veda, when inoculated with California isolates and to introgress this resistance into open-pollinated processing tomato cultivars, developed by the California Tomato Research Institute (CTRI). A standard root-dip method was used to inoculate the seedlings. The evaluation of the plant reactions was based mainly on foliar symptoms - wilting and chlorosis of cotyledons and leaves. A classical backcross strategy was followed to introgress the resistance into CTRI cultivars. The reactions of Veda line (P1), CTRI cultivar 155 VFFNPto (P2), F1, F2, BC1P1 and BC1P2 after inoculation separately with two race 2 isolates led to the conclusion that the factor conferring resistance in the Veda line is dominantly inherited. The level of resistance of F1 plants is comparable to that of the Veda line. BC1P2 segregants showing high level of resistance were selected and the second backcross to 155 VFFNPto was performed.

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