ISHS Acta Horticulturae 693: III International Chestnut Congress
PRELIMINARY DIVERSITY STUDIES OF CULTURABLE PHYLLOSPHERE BACTERIA ON CHESTNUT (CASTANEA SATIVA) |
| Authors: | A. Valverde, J.M. Igual, I. Santa Regina, S. Salazar, C. Rodriguez-Barrueco |
| Keywords: | chestnut, phyllospheric bacteria, diversity, 16S rDNA sequencing |
| Abstract:
In this work we isolated the culturable bacterial community from the phyllosphere of chestnut trees (Castanea sativa) and analysed its fluctuation along a year period. A total of 360 bacterial isolates were collected at different times (June, August and October) from 10 trees. The isolates were grouped into five phenotypes: unpigmented, yellows, oranges, roses and melanin producers. The taxonomic position of 9 isolates belonging to these 5 phenotypes was determined by 16S rDNA sequencing. Five different genera were found among them: Paenibacillus, Sphingomonas, Acinetobacter, Staphylococcus, Bacillus, Klebsiella, and Pseudomonas. This result suggests that the genetic diversity of the bacterial community was very high. | |
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