ISHS Acta Horticulturae 692: II International Symposium on Biotechnology of Tropical and Subtropical Species
THIDIAZURON IN THE IMPROVEMENT OF BANANA MICROPROPAGATION |
| Author: | S.W. Lee |
| Keywords: | Paclobutrazol |
| Abstract:
In 1983 a commercial micropropagation system was established in Taiwan to produce disease free planting material to control the spread of Fusarium wilt (race 4). With the rapid increase in production costs, it was necessary to search for a more active cytokinin than 6-Benzylaminopurine (BA) to improve the efficiency of the micropropagation system. Thidiazuron (TDZ) was tested in the range of 0.002 to 2.0 mg/L for its influence on the proliferation of adventitious buds. TDZ at 0.2 mg/L almost doubled the multiplication rate of Cavendish cultivars in medium containing BA at 4.0 mg/L. The effect of TDZ on the multiplication rate varied among the commercial cultivars 'Pei-Chiao', 'Tai-Chiao No. 1' (AAA) and Latundan (AAB). The multiplication rate of shoots decreased gradually with increasing numbers of subculture cycles. The addition of paclobutrazol (PP333) (0.5 - 1.0 mg/L) to TDZ (0.1 - 0.2 mg/L) resulted in a further increase of proliferation of buds when compared with medium containing only TDZ, while also suppressing shoot elongation. The dwarf bud clusters induced in the TDZ + PP333 medium returned to normal shoots after one to two subcultures in medium containing only BA. The sequence of application of Dropp (containing TDZ as active ingredient) in combination with both BA and PP333 in the micropropagation system is discussed. Tissue culture plantlets produced from adventitious buds treated with TDZ and PP333 grew vigorously during the nursery stage. Field trials of 1,800 plants treated by TDZ showed 2.4% off-types, while the control group consisting of 400 BA treated plants showed 3.0% off-types. | |
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