ISHS Acta Horticulturae 692: II International Symposium on Biotechnology of Tropical and Subtropical Species
APPLICATION OF TISSUE CULTURE TECHNIQUES IN WOODY SPECIES CONSERVATION, IMPROVEMENT AND DEVELOPMENT IN VIETNAM: AGARWOOD (AQUILARIA CRASSNA PIERRE EX LECOMTE) VIA SHOOT-TIP CULTURE |
| Author: | T. Van Minh |
| Keywords: | Agarwood, satalum, resin, micropropagation, tok |
| Abstract:
Aquilaria crassa (agarwood), a Vietnamese forest tree, was micropropagated using shoot explants from 20-year old trees known to produce the valuable exudates ‘tok’. Either shoot tips or internodes could be used for the initial explants, but in subcultures best results were obtained from internodes. Woody Plant Medium was a better basal medium than Murashige and Skoog, and for initial shoot induction BA at 1mg/l and coconut water at 10% was used. For subcultures, BA at 0.1mg/l, NAA 0.1mg/l and coconut water at 10% gave highest shoot multiplication. A low level of rooting was obtained using either IBA or NAA at 0.3 mg/l. Plants transferred to the field grew to 2m after 18 months and had normal morphology. | |
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