ISHS Acta Horticulturae 692: II International Symposium on Biotechnology of Tropical and Subtropical Species
ISOLATION AND CHARACTERIZATION OF CDNAS ENCODING ACC OXIDASE FROM PEPPER |
| Authors: | S.L. Sim, U.K. Abu Bakar, M. Mahmood |
| Keywords: | Delay fruit ripening, Piper nigrum, cDNA library, antisense technology, ethylene |
| Abstract:
A cDNA library of pepper (Piper nigrum L.) was constructed from the pericarp of ripening berries. The cDNAs were cloned into Uni-ZAP XR vector and then packaged into bacteriophage λ. Screening and isolation of the cDNA clones encoding ACC oxidase were carried out using a papaya ACC oxidase cDNA clone as a heterologous probe. Seven putative clones have been isolated and partially sequenced. EMBL database homology searches showed that the partial nucleotide sequences of these putative cDNA clones are similar to the ACC oxidase sequences of other plant species. Characterization and full length sequencing of these clones are in progress. Two of the clones, p2118 and p3232 have already been fully sequenced. The clone p2118 is 1376 bp long consisting of a 110 bp 5’-untranslated region, a predicted 942 bp coding region of 314 amino acids and a 304 bp 3’-untranslated region. Clone p3232 is 1013 bp long, contains a predicted 680 bp coding region encoding 231 amino acids. Both clones showed very high sequence homology to different groups of plant ACC oxidase genes. | |
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