ISHS Acta Horticulturae 692: II International Symposium on Biotechnology of Tropical and Subtropical Species
ADVENTITIOUS SHOOT FORMATION ON TEAK (TECTONA GRANDIS L.F.) CALLUS CULTURES DERIVED FROM INTERNODAL SEGMENTS |
| Authors: | S.N. Widiyanto, D. Erytrina, H. Rahmania |
| Keywords: | Compact-callus, micropropagation, shoot-organogenesis, thidiazuron |
| Abstract:
A procedure was developed to induce shoot organogenesis of teak (Tectona grandis L.f.) callus cultures. Calli were induced from internodal segments on woody plant medium containing 1.0 然 thidiazuron in combination with 0.01 然 indole butyric acid. Results showed that compact callus, with white to light yellow nodular structures on its surface, were produced in four weeks. The most regenerative callus was noted as the compact callus with light yellow or light green color. Shoot organogenesis was induced on medium containing 10.0 然 benzyl adenine in combination with 1.0 然 gibberellic acid. Adventitious shoots proliferated from the surface of regenerative compact callus after three subcultures. Regenerated shoots were propagated on shoot multiplication medium with the addition of 10.0 然 BA. Most elongated shoots were rooted in a soil-sand mixture medium (1:1), and were established under greenhouse conditions. | |
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