ISHS Acta Horticulturae 692: II International Symposium on Biotechnology of Tropical and Subtropical Species
APPLICATION OF TISSUE CULTURE TECHNIQUES IN WOODY SPECIES CONSERVATION, IMPROVEMENT AND DEVELOPMENT IN VIETNAM: MANGOSTEEN (GARCINIA MANGOSTANA L.) VIA EMBRYOGENESIS CULTURE |
| Author: | T. Van Minh |
| Keywords: | Semi-mature, secondary embryogenesis, container, primary leaves |
| Abstract:
MS medium was the most suitable medium for embryogenesis, shoot proliferation and shoot elongation cultures. In embryogenesis cultures, multiple embryos were observed on seed explants cultured on MS medium supplemented with 1mg/l BA, 0.1mg/l NAA and coconut water (10%) after 4-6 weeks of culture. About 80-100 embryos raised on the seed surface and multiple embryos developed to multiple shoots after 2-3 weeks of culture. Secondary embryogenesis continually initiated on the mother embryogenic sample that had 2-3 embryos combined, but did not appear on single embryos. In shoot proliferation cultures, seed with multiple shoots was cut into 4 explants before subculturing on MS medium supplemented with 4mg/l BA, 0.1mg/l NAA, and coconut water (10%). The highest mean number of shoots per explant (18-22) formed after 8 weeks of culture. In shoot elongation cultures, each multiple shoot explant was cut into 4 clumps, having 4-5 shoots per clump, before culturing on MS medium supplemented with 0.1mg/l BA, 0.1 mg/l NAA, 2g/l yeast extract and coconut water (10%). Elongated plants with a height of 20-30mm developed after 4-6 weeks of culture. For rooting, the buds were cultured on WPM medium supplemented with 0.1mg/l BA, 5mg/l IBA, 2g/l yeast extract and coconut water (10%). About 85% of plants produced roots with a root length of 3-4cm and had 1-2 roots per bud after 8 weeks of culture. The healthy plants were transplanted to pots in ex vitro conditions for further studies on their growth characteristics, flowering and fruit quality characteristics. Micropropagation via embryogenesis culture was the best method for production of mangosteen planting materials. | |
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