ISHS Acta Horticulturae 692: II International Symposium on Biotechnology of Tropical and Subtropical Species
GENOTYPE SPECIFIC SOMATIC EMBRYOGENESIS IN SWEETPOTATO |
| Authors: | S.L. Sim, M.J. Cardosa |
| Keywords: | sweetpotato cultivars, in vitro plant regeneration, 2,4-D, Ipomoea batatas |
| Abstract:
Shoot tips of twelve sweetpotato genotypes grown in Sarawak, Malaysia, were excised, each with three to four leaf primodia. The explants were cultured in the dark in a solid MS basal medium supplemented with 2,4-D at 0.5, 1 and 2mg/l. Embryogenesis was successfully induced in genotype Zishu in medium containing 2,4-D at 2mg/l. Though bright-yellow coloured globular structures were also observed in shoot tips cultures of cv. Gendut and genotypes Bukell and Shantou, they were found later to have reverted into the friable and loose non-embryogenic type. No embryogenic calli were found in the cultures of the other eight genotypes. The compact globular embryogenic callus of genotype Zishu developed from the apical dome as well as the leaf primodia. The embryogenic callus developed into globular-and heart-shaped embryos in the induction medium. When transferred to a medium containing ABA at 0.03mg/l and grown under light, cotyledonary embryos developed and turned green. Complete plantlets were obtained in a MS basal medium with both ABA and 2ip supplemented at 0.02mg/l or in basal medium without any plant growth regulators. The conversion rate of complete plantlets from somatic embryos was low. A short period in the dark and growing on medium containing gibberellic acid at 0.05 to 0.1mg/l appeared effective in increasing the production of complete plantlets. | |
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