ISHS Acta Horticulturae 692: II International Symposium on Biotechnology of Tropical and Subtropical Species
EVALUATION OF GRAFT COMPATIBILITY FOR TAXONOMIC RELATIONSHIPS AMONG SPECIES OF THE ORANGE SUBFAMILY |
| Authors: | N. Nito, S.H. Han, Y. Katayama |
| Keywords: | Callus, citrus relatives, germplasm, tissue culture |
| Abstract:
Callus tissues induced from shoots of species in the orange subfamily (Aurantioideae) were grafted in vitro to evaluate the correlation between reaction at the callus graft interface and taxonomic relationship. The graft interface between two pieces of callus from species that are taxonomically close was not distinguishable by anatomical observations. On the other hand, in the combinations of less closely related species, the border of graft interface was distinct. In combinations of species that were even more distant taxonomically, the border was clear and some deposits accumulated. In these combinations the decay of cell wall was evident at the contact surface of both species of callus cells. The response of the grafted callus cells and whether or not incompatibility was evident, was correlated with the taxonomic relationships of the species. | |
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