ISHS Acta Horticulturae 689: VII International Symposium on Grapevine Physiology and Biotechnology
DISEASE RESISTANCE ANALYSES OF TRANSGENIC GRAPEVINES THAT CONTAIN ENDOCHITINASE OR ANTIMICROBIAL PEPTIDE GENES |
| Authors: | J.R. Kikkert, J.R. Vidal, P.G. Wallace, B.I. Reisch, S. Carcia-Zitter, W.F. Wilcox, D.M. Gadoury, R.C. Seem, T.J. Burr |
| Keywords: | Agrobacterium vitis, Botrytis cinerea, chitinase genes, genetic transformation, magainin genes, Uncinula necator, Vitis vinifera |
| Abstract:
Most cultivars of Vitis vinifera are highly susceptible to several diseases. We sought to develop disease resistant forms of important cultivars by genetic engineering with an endochitinase gene (ThEn-42) from Trichoderma harzianum strain P-1; a magainin gene (mag2) from Xenopus laevis, and a synthetic magainin gene (MSI99). Endochitinase-expressing ‘Chardonnay’ and ‘Merlot’ vines were evaluated for resistance to Uncinula necator in the greenhouse and field. Some resistance was observed, but it was not correlated with chitinase expression. Significant resistance to Botrytis cinerea was observed in high endochitinase-expressing ‘Chardonnay’ fruit clusters inoculated in the field. Magainin-expressing lines exhibited a low level reduction of symptoms after inoculation of greenhouse plants with U. necator. However, significant reduction of gall size was obtained after inoculation of magainin-expressing ‘Chardonnay’ lines with Agrobacterium vitis. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
