ISHS Acta Horticulturae 689: VII International Symposium on Grapevine Physiology and Biotechnology
PROGRESS IN BIOINFORMATICS - THE CHALLENGE OF INTEGRATING TRANSCRIPTOMIC, PROTEOMIC AND METABOLOMIC INFORMATION |
| Authors: | G.R. Cramer, J.C. Cushman, D.A. Schooley, D. Quilici, D. Vincent, M.C. Bohlman, A. Ergul, E.A.R. Tattersall, R. Tillett, J. Evans, R. Delacruz, K. Schlauch, P. Mendes |
| Keywords: | Vitis vinifera, abiotic stress, proteins, genes, metabolites, bioinformatics |
| Abstract:
Regulated-deficit irrigation has been used successfully to grow grapes with less water, an important feature in arid regions such as Nevada. An added benefit of water stress is that it affects important aroma, flavor and color constituents in grape berries and wine subsequently made from the fruit. These changes can be associated with improved wine quality and human health benefits. We have initiated an integrative and quantitative analysis of transcript, protein and metabolite changes following abiotic stress imposition in an effort to enhance production efficiency under stressful conditions and to understand the plant-derived contribution to improved wine characteristics. In collaboration with the International Grape Genomics Program community (www.vitaceae.org) we have developed the first Affymetrix GeneChip for Vitis vinifera and related hybrids containing probe sets for approximately 16,000 genes in order to conduct large-scale transcript profiling. In addition, we are using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) coupled with tandem mass spectrometry and gas chromatography-mass spectrometry (GC-MS) to conduct parallel, large-scale protein and metabolite profiles. Custom bioinformatics resources are being developed to integrate experimental results and information about transcript, protein, and metabolite changes that arise in response to abiotic stress exposure. Results from transcript, protein and metabolite profiling from abiotically-stressed leaves from a long-term experiment are summarized. These analyses will be used to elucidate specific metabolic pathways that are perturbed by water deficits and salinity. The comprehensive data sets generated by this project will be critical for our ability to consider engineering strategies for improving wine quality in the future. | |
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