ISHS Acta Horticulturae 683: V International Symposium on New Floricultural Crops
THE TEMPORARY IMMERSION SYSTEM (T.I.S.) FOR THE IMPROVEMENT OF MICROPROPAGATION OF ORNAMENTAL PLANTS |
| Authors: | B. Ruffoni, M. Savona |
| Keywords: | In vitro culture, liquid culture, RITAź, Zantedeschia aethiopica, Anthurium andraeanum |
| Abstract:
The clonal propagation through tissue culture techniques has limitations in the flower industry due to the requirementof highly skilled manual labour and to the limitation of automatia. The growth in liquid medium is an interesting alternative that might provide opportunities only if it will be possible to control or avoid the tissue hyperhydration. Several attempts, with the aims to overcome this negative feature and to increase production rates, were made with a system which provides a temporary contact between the biomass and the liquid medium. Some results were recently reported for tropical cultures, Eucaliptus, Aspen and Psidium guajava and fruit trees (the apple rootstock M26). For this purpose a particular container has been studied by Teisson and Alvard (1995) and registered by CIRAD (France). In this paper micropropagation trials were done with the ornamental plants Zantedeschia aethiopica and Anthurium andraeanum utilising automated containers for temporary immersion. The explants were grown in multiplication media with the composition of the semisolid substrates previously determined without the gelling agent. 8 immersion treatments of 3 min each were programmed for all cultures. The system was assembled in the growth chamber with a photoperiod of 16 h of light at 30 µmol m-2 s-1 of photosynthetic photon flux density (PPFD) and at a temperature of 24°C ± 1. Experiments with the growth regulator benzyladenine and gibberellic acid were conduced. Every 20 days the medium was renewed and the biomass fresh weight and the multiplication rate were recorded. Interesting results in term of proliferation, shoot growth and root emission were obtained for both species. For Zantedeschia it was also possible to avoid the callus formation which was previously observed in continuous liquid culture. The complete plantlets produced in TIS have been also acclimatized promptly. In conclusion both species have good potential to be cultured with this system and to perform the scale up for the industrial production. | |
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