ISHS Acta Horticulturae 683: V International Symposium on New Floricultural Crops
ACTIVATED CHARCOAL FOR IN VITRO PROPAGATION OF BRAZILIAN ORCHIDS |
| Authors: | L.M. Moraes, R.T. Faria, F.L. Cuquel |
| Keywords: | orchidaceae, Laelia flava, Miltonia flavescens, Oncidium trulliferum, culture-media |
| Abstract:
This research focused on the effects of activated charcoal on the in vitro growing media used in the propagation of Brazilian native orchids. Activated charcoal has been described improving the in vitro plant growing and survival after transplant. Seeds were germinated in vitro and after three months they were transferred to one of the following media: MS (Murashige and Skoog) media; half strength of MS media; MS media supplemented with 1 g.L-1 of activated charcoal; half strength of MS media supplemented with 1 g.L-1 of activated charcoal; MS media supplemented with 2 g.L-1 of activated charcoal; half strength of MS media supplemented with 2 g.L-1 of activated charcoal. After six months the following features were evaluated, plantlet height, number of branches per plant, number of roots per plant, length of the roots, fresh weight and O. trulliferum was also evaluated concerning with the pseudobulb diameter. Just after the in vitro plant features evaluation they were transferred to pots containing the tree fern fiber and sphagnum. Two months after transplant the survival rate was evaluated for each treatment. Activated charcoal improved the in vitro plant quality and increased the survival after transplant of the three species evaluated. To M. flavescens and L. flava best results were obtained with half strength of MS media supplemented with 2 g.L-1 of activated charcoal and O. trulliferum with MS media supplemented with 1 g.L-1 of activated charcoal. | |
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