ISHS Acta Horticulturae 682: V International Postharvest Symposium
PROTEOME ANALYSIS OF ORGANOLEPTIC QUALITY IN TOMATO |
| Authors: | M. Rossignol, M. Sommerer |
| Keywords: | Lycopersicon esculentum, fruit quality, QTL-NILs, 2D-gel electrophoresis, mass spectrometry |
| Abstract:
Organoleptic quality of tomato fruits involves among others aroma, taste, colour and texture. To study the genetic bases of these traits, QTL analyses of a recombinant inbred line (RIL) population created from an intraspecific cross between a cherry tomato line and a common tomato line were carried out. QTLs with large effects on fruit weight, locule number and sugar content were detected in the distal 50 cM segment of chromosome 2. Near isogenic lines (NILs) were generated to characterise this particular chromosomal region. A proteomic approach was initiated to investigate characteristics involved in fruit quality at the protein level. Total protein extracts of the tomato pericarp were separated by two-dimensional gel electrophoresis. Two NILs differing in the concerned segment of chromosome 2 as well as the two parental lines of the RIL population were compared during several fruit developmental stages. As expected there are more differences between the parental lines than between the NILs. Several proteins of interest were analysed by MALDI-TOF or ESI-MS/MS and candidate genes for the above mentioned characteristics were obtained. Some of these genes are well known to be linked to fruit development processes but many of them are related to stress and defence response. | |
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