ISHS Acta Horticulturae 682: V International Postharvest Symposium
GENOMIC APPROACHES TO POSTHARVEST BIOTIC AND ABIOTIC STRESSES OF CITRUS FRUIT |
| Authors: | L. González-Candelas, P. Sánchez-Torres, S. Alamar, B. Establés, A.R. Ballester, M.T. Sánchez-Ballesta, Y.L. Luch, M.J. Gosalbes, L. Zacarias, J.F. Marcos, M.T. Lafuente, J. Forment, A. Granell |
| Keywords: | cDNA, chilling injury, EST library, functional genomics, heat conditioning, Penicillium digitatum |
| Abstract:
Citrus fruit may be subjected to biotic and abiotic stresses during postharvest handling and storage that influence their marketability. The major biotic stress is caused by phytopathogenic fungi of the genus Penicillium, being P. digitatum the most important pathogen. Among the abiotic stresses, we are particularly interested in chilling injury and in the heat-induced chilling tolerance of chilling-sensitive citrus cultivars. To gain a deeper knowledge on the mechanisms underlying the defence response of Citrus fruit to pathogen attack and on the heat-induced chilling tolerance, we have undertaken two complementary approaches. In the framework of the Spanish “Citrus Functional Genomic Project, CFGP” we have generated one cDNA library for each condition that will allow us to get a general picture of the genes being transcribed under these conditions. The abiotic stress library was obtained by pooling RNA from the flavedo of mandarin fruits that were heat-conditioned at 37°C for up to three days followed by storage at 2°C for up to ten days. The biotic stress cDNA library was obtained from the rind of orange fruits 24 h after inoculation with P. digitatum. For each library, a total of 1152 clones have been analysed. A more directed approach has been the utilization of the Suppression Subtractive Hybridization technique to generate subtracted cDNAs libraries enriched in those genes preferentially expressed under a particular condition. In this paper we present an overall view of the results of both approaches, with a special emphasis on the common responses found between the two different stresses, and, within each stress, on the comparison between non-selected ESTs versus subtracted libraries. | |
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