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ISHS Acta Horticulturae 682: V International Postharvest Symposium

PROTEOMIC ANALYSIS AND FRUIT RIPENING

Authors:   J.K.C. Rose, M. Saladié
Keywords:   proteomics, tomato fruit ripening, cell wall, genomics, protein sequencing and detection, 2-D gel electrophoresis
DOI:   10.17660/ActaHortic.2005.682.22
Abstract:
The study of complex biological processes, such as fruit ripening, through comparative proteomics is becoming increasingly attractive to plant biologists as the rapidly expanding plant genomic and EST sequence databases provide improved opportunities for protein identification. This review summarizes some typical experimental approaches that are used to evaluate complex protein populations and to identify differentially expressed proteins. In addition a number of practical issues are highlighted that are associated with comparative proteomic analysis including the challenges of effective protein extraction and separation from problematic plant tissues, that often includes fruits, the pros and cons of 2-D gel-based analysis and the challenge of identifying proteins from species that are not recognized models for functional genomic studies.

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