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ISHS Acta Horticulturae 680: III WOCMAP Congress on Medicinal and Aromatic Plants - Volume 6: Traditional Medicine and Nutraceuticals

EFFECT OF STREBLUS ASPER LEAF EXTRACT ON SELECTED ANAEROBIC BACTERIA

Authors:   S. Taweechaisupapong, S. Singhara, T. Choopan
Keywords:   antibacterial effect, disc diffusion, endodontic infection, herbal extract, medicinal plant
Abstract:
The purpose of this in vitro study was to determine the antibacterial effects of leaf extract of koi (Streblus asper) against six anaerobic bacteria: Porphyromonas gingivalis W50, Prevotella intermedia, Actinomyces naeslundii (T14V), Peptostreptococcus micros, Actinobacillus actinomycetemcomitans ATCC 43717 and ATCC 43718. The dried-pulverized leaves were extracted using 50% (v/v) redistilled ethanol. Employing the disc diffusion method, it was demonstrated that 15 µL of the leaf extract at 250 and 500 mg/mL had inhibitory effects towards all bacterial strains tested except A. actinomycetemcomitans ATCC 43717. The minimum inhibitory concentration of S. asper leaf extract on P. gingivalis W50, A. actinomycetemcomitans ATCC 43717 and ATCC 43718, P. micros, P. intermedia, and A. naeslundii (T14V) were 3.9, 31.3, 7.8, 15.6, 31.3, and 125 mg/mL respectively. The minimum bactericidal concentration of the extract on P. gingivalis W50, P. micros, A. actinomycetemcomitans ATCC 43717 and ATCC 43718, were 31.3, 62.5, 62.5 and 15.6 mg/mL respectively. The extract had no bactericidal activity against P. intermedia and A. naeslundii (T14V).

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