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ISHS Acta Horticulturae 680: III WOCMAP Congress on Medicinal and Aromatic Plants - Volume 6: Traditional Medicine and Nutraceuticals

FREE RADICAL SCAVENGING AND TYROSINASE INHIBITION ACTIVITY OF AROMATIC VOLATILE OIL FROM THAI MEDICINAL PLANTS FOR COSMETIC USES

Authors:   A. Manosroi, J. Manosroi
Keywords:   antiwrinkle, betel vine, holy basil, kaffir lime, kojic acid, vitamin C, vitamin E, whitening
Abstract:
Free radical scavenging and tyrosinase inhibition activity of thirteen aromatic oils from Thai medicinal plants were investigated. A stable free radical DPPH (1, 1-diphenyl-2-picryhydrazyl) was used in the free radical scavenging assay and mushroom tyrosinase enzyme was used in the tyrosinase inhibition test. The two assays were performed in 96-well plates and the absorbance measured by a well reader. Betel vine and holy basil oils gave the highest free radical scavenging activity with percent residual rate of absorbance (%RRA) of 15%, which is comparable to vitamin E and vitamin C, widely used in antiwrinkle cosmetics. Kaffir lime leaf and fruit peel oils showed the highest tyrosinase inhibition activity of 86.8 and 100%, respectively, which is similar in potency to kojic acid, a popular ingredient in whitening cosmetics. This study demonstrated the potential use of aromatic volatile oils extracted from Thai medicinal plants in commercial cosmetics.

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