ISHS Acta Horticulturae 678: III WOCMAP Congress on Medicinal and Aromatic Plants - Volume 4: Targeted Screening of Medicinal and Aromatic Plants, Economics and Law
NOVEL WATER SOLUBLE ANTIBACTERIAL DRESSING OF DURIAN POLYSACCHARIDE GEL |
| Authors: | S. Pongsamart, N. Nanatawanit, J. Lertchaipon, V. Lipipun |
| Keywords: | antibacterial dressing, durian gel dressing, Durio zibethinus L., wound dressing |
| Abstract:
Polysaccharide gel (PG) extract of fruit-hulls of durian (Durio zibethinus L.) was previously analyzed its sugars component that identical to 4 standard sugars; arabinose, rhamnose, fructose, glucose and galacturonic acid, respectively. PG exhibited antibacterial activity. In vitro study was performed to evaluate the susceptibility of bacteria by agar diffusion test. PG at 0.32% concentration showed sharp and clear inhibition zones on MHA plate against Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus, Bacillus subtilis and Escherichia coli; and on BHIA plate against Lactobacillus pentosus. Antibacterial activity of PG was determined by broth macrodilution method. The colony counts of M. luteus and S. aureus declined to 0% in the presence of 2.5% PG at 16 and 20 hours, respectively; and colony count of L. pentosus declined to 71.28% at 24 hours. Minimal inhibitory concentrations (MICs) of PG were 6.4 mg/ml against B. subtilis, E. coli, S. epidermidis and M. luteus; 12.8 mg/ml against S. aureus and 25.6 mg/ml against L. pentosus. Minimal bactericidal concentrations (MBCs) of PG were 25.6 mg/ml against B. subtilis, E. coli, S. aureus, S. epidermidis and M. luteus; and 51.2 mg/ml against L. pentosus. PG was also demonstrated the killing effect against S. aureus and E. coli at 0.02% PG in NSS, colony counts were decreased to zero within 24 hours. Scanning electron microscopy showed an appearance of bacterial cells aggregation and adhesion of PG fragments on cell surface. Preparation of film dressing of PG was successfully prepared with the presence of plasticizer, 10% propylene glycol or 15% glycerin, to produce a satisfactory PG film dressing product by a casting/solvent evaporation method. Soft fiber of PG dressing patch was also prepared by freeze drying a solution of 0.5% PG in water. PG film showed antibacterial effect against susceptible bacteria tested. The results suggest that preparation of PG dressing has potential benefit in using as a new water soluble antibacterial dressing for open wounds. | |
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