|Authors: ||T. Punjanon, P. Nandhasri|
|Keywords: ||noni, antinoniceptive effect, writhing test, abdominal constriction, morphine, acetic acid|
The alcoholic extract from the fruits of Morinda citrifolia (noni) was evaluated for analgesic effect in mice using the acetic acid-induced writhing test.
The extract was administered by i.p. injection in doses of 1, 2, 3 and 4 g of dried plant material kg-1 of animal body weight, 15 min before i.p. injection of acetic acid (0.75%). Morphine sulfate (1.5 mg kg-1, i.p.) was used as the reference drug.
Control animals received i.p. injections of 0.9% NaCl solution, instead of the extract.
In control mice, the number of writhes during the 20 min test period was 43.0 ± 1.4 (mean ± S.E.M., n=6). The extracts produced a significant dose-dependent inhibition of acetic acid-induced abdominal constriction.
The percentage inhibition was 4.4 ± 4.5, 21.2 ± 11.2, 71.4 ± 5.0, 93.1 ± 1.7 (mean ± S.E.M., n=6) at the doses of 1, 2, 3 and 4 g kg-1 respectively, compared to control animals.
The inhibitory effect of the 4 g kg-1 dose of extract was similar to that produced by morphine in a dose of 1.5 mg kg-1. The antinoniceptive effect in writhing test was statistically significant (p<0.001) for 15 min until 5 hr of administration.
The data obtained suggest that the alcoholic extraction from the fruits of Morinda citrifolia appears to have analgesic effect.
Further studies are necessary for the identification of the active principles and more detailed elucidation of its mechanism of action is required.
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