ISHS Acta Horticulturae 677: III WOCMAP Congress on Medicinal and Aromatic Plants - Volume 3: Perspectives in Natural Product Chemistry
CHIEF CONSTITUENTS AND BIOLOGICAL ACTIVITIES DETERMINATION OF STEPHANIA VENOSA |
| Authors: | B. Potduang, T. Kajsongkram, P. Limsiriwong, R. Giwanon, K. Thisayakorn, M. Meeploy, Y. Benmart |
| Keywords: | Stephania venosa, Artemia sp., TLC fingerprints, antioxidant, anti-tyrosinase, anti-inflammation, anti-microbial |
| Abstract:
Accessible utilization study of Stephania venosa was carried out on TLC fingerprints and various biological activity tests. Chief constituents in tuberous roots extractives of S. venosa were detected on TLC fingerprints as 3 different zones of steroids and terpenes, 8 different zones of alkaloids, 6 different zones of flavonoids and 2 different zones of phenol carboxylic acids as indicated by the hRf values and visualized colours resulting from spraying with special detection reagents. It was found that ethanol extract from tuberous roots of S. venosa displayed marked inhibitory activity on Artemia sp. with the LC50 of 260.26 µg/ml. The extract exhibited antioxidant activity with the EC50 of 4.98 µg/ml by DPPH radical scavenging assay. The microplate assay using the Dopachrome method indicated that the extract inhibited mushroom tyrosinase with the IC50 of 1.74 mg/ml. The extract given at the dose of 250 mg/kg orally, presented inconsistent anti-inflammatory activity as an antioedematogenic effect in rat paw oedema induced by carrageenin only at 1 h (74.3%), and the activity was reduced thereafter; toxic/lethal dose of the extract was 750 mg/kg with anticholinesterase-like effects. The agar dilution method assay showed that the extract was active against various microbial enteropathogens and dermatopathogens, with the minimum inhibitory concentrations (MICs) between 5-30 mg/ml. | |
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