ISHS Acta Horticulturae 677: III WOCMAP Congress on Medicinal and Aromatic Plants - Volume 3: Perspectives in Natural Product Chemistry
ANTI-SNAKE VENOM EFFECT OF ARISTOLOCHIA ODORATISSIMA L. AQUEOUS EXTRACT ON MICE |
| Authors: | A. Usubillaga, N. Khouri, S. Cedillo-Vaz, E. Yibirin |
| Keywords: | Aristolochiaceae, aristolochic acid I, Bothrops atrox, (-)-cubebin, infusion |
| Abstract:
In the low lands south of Maracaibo Lake people drink an infusion of Aristolochia odoratissima leaves to treat snakebites. A preliminary study was made to determine the toxicity of the venom used. A dose of 16 mg/kg produced 100% mortality in 30 min. To test the efficacy of the plant, doses of 60, 40, 30, and 20 mL of A. odoratissima aqueous extract obtained from 30 g of leaves in 150 mL of water, were given to 4 groups of 40 mice 96 hours before i.p. injection of Bothrops atrox venom. It was observed that animals that received orally the higher doses of plant extract (60 and 40 mL) protected against the highest dose of venom (16 mg/kg) since mortality descended from 100% to 80%. On the other hand when the aqueous extract was given i.p., no favorable effects were observed. Extraction of dried roots with pentane and leaves with hexane permitted to isolate the lignan (-)-cubebin. Aristolochic acid I was obtained by extraction with EtOH. | |
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