ISHS Acta Horticulturae 676: III WOCMAP Congress on Medicinal and Aromatic Plants - Volume 2: Conservation, Cultivation and Sustainable Use of Medicinal and Aromatic Plants
EFFECT OF SALINITY AND NITROGEN NUTRITION ON FREE PROLINE AND SEED OIL CONTENT OF MEDICINAL PUMPKIN (CUCURBITA PEPO SUBSP. PEPO CONVAR. PEPO VAR. STYRIACA) |
| Authors: | H. Aroiee, M. Azizi, R. Omibaigi |
| Keywords: | osmolyt compound, naked seed pumpkin, environmental stress |
| Abstract:
Field and greenhouse studies were conducted in 1998 and 1999 to study the effect of salinity and nitrogen nutrition on the amount of free proline in the leaves and the seed oil content of common pumpkin. Salinity treatments were applied at four levels (0, 2.5, 5, and 10 g.l-1 of NaCl) and nitrogen nutrition treatments were applied at five levels (0, 75, 150, 225, and 300 kg.ha-1 of NH4NO3). In this research leaf dry weight, N concentration of different plant parts, leaf proline content, K:Na ratio of the leaf and root, and seed oil content of the plants were determined. Salinity stress was applied at two stages i.e., by soaking the seeds in the solution for 36 h before planting, and during growth stage from four leaf stage until flowering. Nitrogen fertilizer was applied at planting, fourth leaf stage, and flowering. The results indicated that in the two years of experiment, salinity stress increased the amount of free proline in the leaves compared to the control. The highest amount of proline was obtained when salinity and nitrogen levels were at 2.5 g.l-1 of NaCl and 225 kg.ha-1 of nitrogen. The highest seed oil content was obtained at salinity of 2.5 g.l-1 of NaCl. Leaf dry weight and leaf and root K:Na ratios decreased as salinity decreased. Leaf N content increased as salinity and nitrogen increased. Oil seed content was highest at 2.5 g.l-1 of NaCl and 75 kg.ha-1 of nitrogen. | |
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