ISHS Acta Horticulturae 676: III WOCMAP Congress on Medicinal and Aromatic Plants - Volume 2: Conservation, Cultivation and Sustainable Use of Medicinal and Aromatic Plants
AN EFFICIENT IN VITRO PROPAGATION OF ZANTHOXYLUM PIPERITUM |
| Author: | S.J. Hwang |
| Keywords: | Zanthoxylum piperitum DC., shoot-tip culture, in vitro propagation |
| Abstract:
A protocol is described for rapid multiplication of Zanthoxylum piperitum DC. (Rutaceae), an important aromatic and medicinal plant, through shoot-tip explant cultures. Murashige and Skoog (MS) medium supplemented with various concentrations of N-6-benzyladenine(BA), N-benzyl-9-(2-tetrahydropyrenyl) adenine (BPA) and thidiazuron (TDZ), either single or in combination with α-naphthaleneacetic acid (NAA), was used to determine the rate of shoot proliferation. N-6-benzyladenine (BA) used at 0.5 mg l-1 was the most effective in initiating shoot proliferation, producing an average of 23 microshoots per shoot-tip explant after 40 days of culture. Induction of rooting (98%) was achieved by transferring the shoots to the same basal medium containing 2 mg l-1 indole-3-butyric acid (IBA). The plantlets were transferred to soil after acclimatization and showed 63% survival. These results indicate that possible application for the mass production of plantlets is through in vitro culture system in Zanthoxylum piperitum DC. | |
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