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ISHS Acta Horticulturae 675: III WOCMAP Congress on Medicinal and Aromatic Plants - Volume 1: Bioprospecting and Ethnopharmacology

CHROMOSOME NUMBERS OF THAI HERBS IN THE PLUMBAGO

Author:   C. Indananda
Keywords:   Ceylon leadwort, mitotic and meiotic chromosomes, rose-coloured leadwort
Abstract:
In Thailand, there are only two known types of herbs in the Plumbago genus: Ceylon leadwort (P. zeylanica) and rose-coloured leadwort (P. indica). They are at risk of becoming extinct due to the difficulty in propagation. This leads to a need for the study of their chromosome numbers for database storage. In this study, the root tips and inflorescences were investigated by using the aceto-carmine smear method. It was found that P. zeylanica and P. indica have different numbers of chromosomes, which are 2n = 22 and 2n = 14, respectively. Because the size of metaphase chromosomes is extremely small, the study of the chromosome numbers needs to be done from the microsporocyte in the anther by collecting inflorescences between November and January. For P. zeylanica, 11 bivalent chromosomes were found in the first meiotic metaphase. P. indica, 7 chromosomes were found in the second meiotic metaphase.

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