ISHS Acta Horticulturae 675: III WOCMAP Congress on Medicinal and Aromatic Plants - Volume 1: Bioprospecting and Ethnopharmacology
DIFFERENTIAL GENE EXPRESSION IN PAPAVER-SPECIES IN COMPARISON WITH ALKALOID PROFILES |
| Authors: | J. Ziegler, T.M. Kutchan |
| Keywords: | benzylisoquinoline, expressed sequence tag, macroarray, morphine, opium poppy |
| Abstract:
Papaver species are known to produce a large variety of benzylisoquinoline alkaloids, with each species exhibiting a specific alkaloid profile, but only a few genes involved in the biosynthesis or regulation of these complex pathways are known so far. Here we used a genomic approach to discover genes responsible for the determination of a specific alkaloid profile. A stem expressed sequence tag database of ~1100 unique genes from Papaver somniferum was created. Gene expression analysis of these sequences in P. bracteatum, P. somniferum and P. somniferum ‘Noscapine’ exhibited 39 cDNAs showing differential expression coincident with morphine accumulation. | |
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