ISHS Acta Horticulturae 673: IX International Symposium on Flower Bulbs
FACTORS INFLUENCING MICROPROPAGATION OF NARCISSUS |
| Authors: | D. Sochacki, T. Orlikowska |
| Keywords: | Narcissus L., contamination, regeneration, multiplication efficiency, bulbil formation |
| Abstract:
The aim of the experiments was to improve the micropropagation protocol for narcissus (Narcissus L.) in three respects to achieve: a decrease in contamination of initial explants, an increase in regeneration and multiplication efficiency, and shortening of the bulbil formation period. For the cultivar ‘Carlton’, a temperature treatment of bulbs (44.4°C for 3 h) in hot water and for ‘Heweliusz’ hot 1% aqueous solution of imazalil (Fungaflor 200), followed in either case by sterilization of bulb segments in 0.1% mercuric chloride and 0.75% chloramine T, resulted in a reduction of the initial contamination to the level of 14 and 17%, respectively. Prolongation of the regeneration stage up to 24 weeks significantly increased the number of regenerated shoots but the most intensive regeneration period depended on the genotype. An increase in the multiplication rate was achieved by cutting bulbils into four parts. The number of shoots varied from 9.2 to 13.1 shoots per 1/4 of bulbil, depending on the concentration of growth regulators. The duration of the bulbil formation period was shortened by 4 weeks by reducing incubation time from eight to four weeks at 20/25°C and increasing the cooling temperature from 5 to 8°C. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
