ISHS Acta Horticulturae 673: IX International Symposium on Flower Bulbs
COMPARISON OF POLLEN MORPHOLOGY IN INTERSPECIFIC HYBRID LILIES AFTER IN VITRO CHROMOSOME DOUBLING |
| Authors: | H.K. Rhee, H.R. Cho, K.J. Kim, K.S. Kim |
| Abstract:
In order to overcome the pollen sterility of interspecific hybrids, in vitro chromosome doubling was conducted. Low concentration (0.001-0.003%, 3 hrs) of oryzalin was more effective than high concentration (0.1%, 3 hrs) of colchicine to obtain tetraploidy. The identification of tetraploids was detected by flow cytometry. The pollen fertility of interspecific hybrid lily was recovered up to 40% in tetraploid level. Pollen grains of the FA96-4 hybrid were malformed. In FA96-18, the pollen length was 67.5 µm in haploid and 107.3 μm in diploid. Most sterile pollen grains were small with an empty shell-like shape and fertile pollen grains were normal with the ‘full oval’ shape. The pollen structure and exine patterns of interspecific hybrids and their parents were observed using SEM microscope. Pollen structure of parents had normal exine structure consisting of connected tactum, bacular, and narrow lumina. The tactum structure of parent’s pollen had net structure, which looked like water flow, while that of most interspecific hybrids was destroyed, resulting in grain-like substances. | |
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