ISHS Acta Horticulturae 673: IX International Symposium on Flower Bulbs
RAPID ENLARGEMENT OF LILY BULBLET BY BIOREACTOR CULTURE |
| Authors: | D.H. Goo, J.H. Lim, H.R. Cho, Y.J. Kim, K.W. Kim |
| Keywords: | Asiatic lily, sucrose concentration, charcoal, ABA, t-zeatin, starch |
| Abstract:
This study was conducted to investigate the optimal culture condition of lily bulblets in a bioreactor. Asiatic hybrid ‘Mirr’ was used to analyze the effects of growth regulator, sucrose concentration, and charcoal on the endogenous contents of ABA, t-zeatin, starch, and starch accumulation in bulblets. Treatment with growth regulator, BA 0.1 mg L-1 and IAA 0.1 mg L-1, resulted in the highest bulblet proliferation in liquid culture with 5.8 bulblets per scale. Addition of 6% sucrose with 0.1% charcoal to the MS medium showed three times higher bulblet enlargement with 8.4 g per bulblet, compared to 3% sucrose alone at 2.7 g/bulblet. Starch content was higher with 9% sucrose with 0.1% charcoal, and accumulation of starch in the cell showed subsequent increases with sucrose concentration in the medium. The starch accumulation was higher in solid agar medium than in the liquid medium. ABA content was lower at 3% sucrose, than other concentrations. This was probably due to a relationship between the bulblet enlargement and endogenous ABA content. The t-zeatin content was highest in the 6% sucrose and 0.1% charcoal treatment. A liquid culture method using an Ebb & flow system resulted in better bulblet enlargement, especially in 6% sucrose treatments, than the ordinary liquid culture method. | |
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