ISHS Acta Horticulturae 673: IX International Symposium on Flower Bulbs
CHARACTERIZATION OF GENES RELATED TO HYACINTH BULB FORMATION INDUCED BY LOW TEMPERATURE OR ABA |
| Authors: | K. Li-Nagasuga, H. Okubo |
| Keywords: | abscisic acid, bulb formation, dormancy, hyacinth-low temperature responsive gene |
| Abstract:
Molecular biological analysis was carried out to clarify the relationship between the induction of bulb formation and the induction of bulb dormancy in geophytes. Gene fragments induced by low temperature or ABA during bulb formation in hyacinth (Hyacinthus orientalis L.) have been obtained with differential display. cDNA clones were obtained from 18 specific gene fragments induced by low temperature or ABA, and they were designated as HL clones. The partial sequences of the HL clones clarified that eight of the 18 (two by low temperature and by ABA and six by low temperature only) HL clones had over 80% identity with identified sequences with FASTA program. Six HL clones of cDNA induced by low temperature and by ABA were homologous to genome DNA of Arabidopsis thaliana or Oryza sativa. Three HL clones showed partial identity with starch phosphorylase gene of Ipomoea batatas. Some HL clones were homologous to genes or mRNAs of which the function had been suggested. It is also possible that HL clones of which the functions were not clarified are novel genes related to bulb formation. | |
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