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ISHS Acta Horticulturae 673: IX International Symposium on Flower Bulbs

CROSS-COMPATIBILITY IN INTERSPECIFIC AND INTERGENERIC HYBRIDIZATION AMONG THE COLCHICACEOUS ORNAMENTALS, GLORIOSA SPP., LITTONIA MODESTA AND SANDERSONIA AURANTIACA

Authors:   S. Kuwayama, Y. Mizuta, M. Nakano, T. Nakamura, T. Oomiya
Keywords:   cross-pollination, FCM analysis, ovule culture, rhizome-like structures, wide hybridization
DOI:   10.17660/ActaHortic.2005.673.53
Abstract:
Interspecific and intergeneric cross-pollination and subsequent ovule culture were carried out among 6 genotypes of Gloriosa spp., 1 genotype of L. modesta and 2 genotypes of S. aurantiaca in order to widen their variations and to develop novel cultivars. Ovules with placental tissues prepared from ovaries 14 days after pollination were cultured at 25C in the dark on a medium containing 0.01 mg l-1 alpha-naphthaleneacetic acid (NAA) and 0.01 mg l-1 benzyladenine (BA), on which rhizome-like structures were produced from the ovules in 49 out of 61 cross-combinations. Following transfer to a medium containing 0.25 mg l-1 NAA and 2.5 mg l-1 BA under a 16-h photoperiod, some of the rhizome-like structures produced shoots and/or root-like structures. These shoots developed into plantlets and produced tubers on a plant growth regulator-free medium. In some of the plantlets derived from intergeneric cross-pollination, early confirmation of the hybridity was accomplished by flow cytometry analysis of relative DNA content of nuclei.

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