ISHS Acta Horticulturae 673: IX International Symposium on Flower Bulbs
CROSS-COMPATIBILITY IN INTERSPECIFIC AND INTERGENERIC HYBRIDIZATION AMONG THE COLCHICACEOUS ORNAMENTALS, GLORIOSA SPP., LITTONIA MODESTA AND SANDERSONIA AURANTIACA |
| Authors: | S. Kuwayama, Y. Mizuta, M. Nakano, T. Nakamura, T. Oomiya |
| Keywords: | cross-pollination, FCM analysis, ovule culture, rhizome-like structures, wide hybridization |
| DOI: | 10.17660/ActaHortic.2005.673.53 |
| Abstract:
Interspecific and intergeneric cross-pollination and subsequent ovule culture were carried out among 6 genotypes of Gloriosa spp., 1 genotype of L. modesta and 2 genotypes of S. aurantiaca in order to widen their variations and to develop novel cultivars. Ovules with placental tissues prepared from ovaries 14 days after pollination were cultured at 25°C in the dark on a medium containing 0.01 mg l-1  -naphthaleneacetic acid (NAA) and 0.01 mg l-1 benzyladenine (BA), on which rhizome-like structures were produced from the ovules in 49 out of 61 cross-combinations.
Following transfer to a medium containing 0.25 mg l-1 NAA and 2.5 mg l-1 BA under a 16-h photoperiod, some of the rhizome-like structures produced shoots and/or root-like structures.
These shoots developed into plantlets and produced tubers on a plant growth regulator-free medium.
In some of the plantlets derived from intergeneric cross-pollination, early confirmation of the hybridity was accomplished by flow cytometry analysis of relative DNA content of nuclei.
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